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vectashield antifade mounting medium with dapi  (Vector Laboratories)


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    Structured Review

    Vector Laboratories vectashield antifade mounting medium with dapi
    Vectashield Antifade Mounting Medium With Dapi, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 98/100, based on 21980 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vectashield+mounting+medium/VECTASHIELD+Antifade+Mounting+Medium+with+DAPI/custom%40h-1200%4042755103
    Average 98 stars, based on 21980 article reviews
    vectashield antifade mounting medium with dapi - by Bioz Stars, 2026-09
    98/100 stars

    Images

    Related Articles

    Staining:

    Article Title: Stimulation to secrete insulin induces pancreatic β-cell dysfunction through Tfe3 activation
    Article Snippet: .. The stained whole islets were mounted on glass slides using VECTASHIELD mounting medium (Vector Laboratories, H-1000), and images were acquired using a confocal microscope (Leica TCS SP8) with Leica Application Suite (v2.0.1.14392) software. .. MIN6 cells were seeded into 48-well plates and reverse transfected with 0.4 μg of the Firefly luciferase reporter plasmid, 0.2 μg of the Renilla luciferase expression plasmid phRG-TK (Promega), and 0.2 μg of the expression plasmid for Tfe3 and its derivatives (total 0.8 μg) using Lipofectamine 3000 reagent (Thermo Fisher Scientific).

    Article Title: Ventral skull based approach to whole-brain extraction in mice
    Article Snippet: .. Sections were stained with DAPI, mounted onto glass slides, air-dried, and coverslipped with Vectashield mounting medium (Vector Laboratories). .. Imaging was performed on an Olympus MVX10 stereo microscope with a 2 × and 0.63 × objective lens, 1.8 × zoom, 0.5 numerical aperture, and 500 ms exposure under 455 nm illumination.

    Microscopy:

    Article Title: Stimulation to secrete insulin induces pancreatic β-cell dysfunction through Tfe3 activation
    Article Snippet: .. The stained whole islets were mounted on glass slides using VECTASHIELD mounting medium (Vector Laboratories, H-1000), and images were acquired using a confocal microscope (Leica TCS SP8) with Leica Application Suite (v2.0.1.14392) software. .. MIN6 cells were seeded into 48-well plates and reverse transfected with 0.4 μg of the Firefly luciferase reporter plasmid, 0.2 μg of the Renilla luciferase expression plasmid phRG-TK (Promega), and 0.2 μg of the expression plasmid for Tfe3 and its derivatives (total 0.8 μg) using Lipofectamine 3000 reagent (Thermo Fisher Scientific).

    Article Title: Histone Density and Dynamics Shape Mitotic Chromatid Architecture in Xenopus Egg Extracts
    Article Snippet: .. After being counter‐stained with DAPI, the coverslips were mounted on slides with VectaShield mounting medium (Vector Laboratories, H‐1000), sealed with nail polish, and processed for fluorescence microscopy. .. Fluorescence microscopy was carried out using a DeltaVision microscope system (Cytiva), which consisted of an inverted fluorescence microscope (IX71, Olympus) equipped with a UPlanSApo 100×/1.40 oil‐immersion lens and a sCMOS camera (pco.edge 4.2 m, PCO AG).

    Software:

    Article Title: Stimulation to secrete insulin induces pancreatic β-cell dysfunction through Tfe3 activation
    Article Snippet: .. The stained whole islets were mounted on glass slides using VECTASHIELD mounting medium (Vector Laboratories, H-1000), and images were acquired using a confocal microscope (Leica TCS SP8) with Leica Application Suite (v2.0.1.14392) software. .. MIN6 cells were seeded into 48-well plates and reverse transfected with 0.4 μg of the Firefly luciferase reporter plasmid, 0.2 μg of the Renilla luciferase expression plasmid phRG-TK (Promega), and 0.2 μg of the expression plasmid for Tfe3 and its derivatives (total 0.8 μg) using Lipofectamine 3000 reagent (Thermo Fisher Scientific).

    RNAscope:

    Article Title: Regulatory elements in the Sox9 locus license the initiation of pancreatic ductal adenocarcinoma.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER hGastrin I Sigma Aldrich Cat#G9145 mNoggin Preprotech Cat#250-38-100UG N-acetylcysteine Sigma Aldrich Cat#A9165 Nicotinamide Sigma Aldrich Cat#N0636 Primocin Invivogen Cat#iant-pm-2 hR-spondin 3 Life Technologies Cat#120-44-1MG FGF10 Preprotech Cat#100-26 mR-Spondin-1 R&D Systems Cat#3474-RS DNAseI from bovine pancreas Sigma Aldrich Cat#11284932001 Soybean trypsin inhibitor Sigma Cat# T6414-6X100ML Fetal Bovine Serum Gibco Cat#17964671 PenStrep Gibco Cat#15-140-122 Lipopolysaccharide (LPS) Enzo Life Sciences Cat#581-013-L001 Phorbol 12-myristate 13-acetate (PMA) Thermo Fisher Scientific Cat# P8139-1MG NutriStem hPSC XF Medium Biological Industries Israel Beit-Haemek Ltd. Cat#05-100-1A Accutase Thermo Fisher Scientific Cat#A1110501 TrypleE Thermo Fisher Scientific Cat#12604013 Dispase Sigma-Aldrich Cat#D4693-1G ROCK inhibitor Y-27632 VWR Cat#688000-100MG H&E Abcam Cat# ab245880 Alcian blue Vector Laboratories Cat#H-3501 Entellan EMD Millipore Cat#1.07960.0500 Vectashield Mounting medium Vector Laboratories Cat# H-1000 Metal Enhanced DAB Substrate Kit Thermo Scientific Cat# 34065 Dnase I Invitrogen Cat#18068015 Dynabeads G ThermoFisher Cat#10003D Critical commercial assays TruChip Chromatin Shearing Kit Covaris Cat#520154 Human Chemokine Array Abcam Cat#ab169812 Rnascope Multiplex Fluorescent v2 ACD Cat#323110 RNeasy Mini Kit Qiagen Cat#74106 RNeasy Plus Micro Kit Qiagen Cat#74034 GoScript Reverse Transcription System kit Promega Cat#A5001 PrimeScriptTM RT Master Mix Takara Bio Cat#RR036A RealQ Plus Master Mix Green Ampliqon Cat# A324406 Paraformaldehyde (PFA) VWR Chemicals Cat#9713-1000 PierceTM Peroxidase Suppressor Thermo Scientific Cat#35000 Collagenase Sigma-Aldrich Cat# C9407-1G Deposited data E15.5 RNA sequencing This paper GEO: GSE316458 Super-enhancer annotation, Smca1, CTCF, Med1 Chip-seq on human islets of Langerhan DOI: 10.1038/s41588-019-0457-0 EGA: EGAS00001002917 ATAC seq of Panc-1 cells DOI: 10.1186/s13045-021-01131-0 GEO: GSE149103 H3K27ac and H3K4me3 Chip-seq on Panc-1 cells DOI: 10.1038/nature11247 (SYDH HistoneP, ENCODE) GEO:GSM818826 (Continued on next page) 18 Cell Reports 45, 117176, April 28, 2026 ..

    Multiplex Assay:

    Article Title: Regulatory elements in the Sox9 locus license the initiation of pancreatic ductal adenocarcinoma.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER hGastrin I Sigma Aldrich Cat#G9145 mNoggin Preprotech Cat#250-38-100UG N-acetylcysteine Sigma Aldrich Cat#A9165 Nicotinamide Sigma Aldrich Cat#N0636 Primocin Invivogen Cat#iant-pm-2 hR-spondin 3 Life Technologies Cat#120-44-1MG FGF10 Preprotech Cat#100-26 mR-Spondin-1 R&D Systems Cat#3474-RS DNAseI from bovine pancreas Sigma Aldrich Cat#11284932001 Soybean trypsin inhibitor Sigma Cat# T6414-6X100ML Fetal Bovine Serum Gibco Cat#17964671 PenStrep Gibco Cat#15-140-122 Lipopolysaccharide (LPS) Enzo Life Sciences Cat#581-013-L001 Phorbol 12-myristate 13-acetate (PMA) Thermo Fisher Scientific Cat# P8139-1MG NutriStem hPSC XF Medium Biological Industries Israel Beit-Haemek Ltd. Cat#05-100-1A Accutase Thermo Fisher Scientific Cat#A1110501 TrypleE Thermo Fisher Scientific Cat#12604013 Dispase Sigma-Aldrich Cat#D4693-1G ROCK inhibitor Y-27632 VWR Cat#688000-100MG H&E Abcam Cat# ab245880 Alcian blue Vector Laboratories Cat#H-3501 Entellan EMD Millipore Cat#1.07960.0500 Vectashield Mounting medium Vector Laboratories Cat# H-1000 Metal Enhanced DAB Substrate Kit Thermo Scientific Cat# 34065 Dnase I Invitrogen Cat#18068015 Dynabeads G ThermoFisher Cat#10003D Critical commercial assays TruChip Chromatin Shearing Kit Covaris Cat#520154 Human Chemokine Array Abcam Cat#ab169812 Rnascope Multiplex Fluorescent v2 ACD Cat#323110 RNeasy Mini Kit Qiagen Cat#74106 RNeasy Plus Micro Kit Qiagen Cat#74034 GoScript Reverse Transcription System kit Promega Cat#A5001 PrimeScriptTM RT Master Mix Takara Bio Cat#RR036A RealQ Plus Master Mix Green Ampliqon Cat# A324406 Paraformaldehyde (PFA) VWR Chemicals Cat#9713-1000 PierceTM Peroxidase Suppressor Thermo Scientific Cat#35000 Collagenase Sigma-Aldrich Cat# C9407-1G Deposited data E15.5 RNA sequencing This paper GEO: GSE316458 Super-enhancer annotation, Smca1, CTCF, Med1 Chip-seq on human islets of Langerhan DOI: 10.1038/s41588-019-0457-0 EGA: EGAS00001002917 ATAC seq of Panc-1 cells DOI: 10.1186/s13045-021-01131-0 GEO: GSE149103 H3K27ac and H3K4me3 Chip-seq on Panc-1 cells DOI: 10.1038/nature11247 (SYDH HistoneP, ENCODE) GEO:GSM818826 (Continued on next page) 18 Cell Reports 45, 117176, April 28, 2026 ..

    Reverse Transcription:

    Article Title: Regulatory elements in the Sox9 locus license the initiation of pancreatic ductal adenocarcinoma.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER hGastrin I Sigma Aldrich Cat#G9145 mNoggin Preprotech Cat#250-38-100UG N-acetylcysteine Sigma Aldrich Cat#A9165 Nicotinamide Sigma Aldrich Cat#N0636 Primocin Invivogen Cat#iant-pm-2 hR-spondin 3 Life Technologies Cat#120-44-1MG FGF10 Preprotech Cat#100-26 mR-Spondin-1 R&D Systems Cat#3474-RS DNAseI from bovine pancreas Sigma Aldrich Cat#11284932001 Soybean trypsin inhibitor Sigma Cat# T6414-6X100ML Fetal Bovine Serum Gibco Cat#17964671 PenStrep Gibco Cat#15-140-122 Lipopolysaccharide (LPS) Enzo Life Sciences Cat#581-013-L001 Phorbol 12-myristate 13-acetate (PMA) Thermo Fisher Scientific Cat# P8139-1MG NutriStem hPSC XF Medium Biological Industries Israel Beit-Haemek Ltd. Cat#05-100-1A Accutase Thermo Fisher Scientific Cat#A1110501 TrypleE Thermo Fisher Scientific Cat#12604013 Dispase Sigma-Aldrich Cat#D4693-1G ROCK inhibitor Y-27632 VWR Cat#688000-100MG H&E Abcam Cat# ab245880 Alcian blue Vector Laboratories Cat#H-3501 Entellan EMD Millipore Cat#1.07960.0500 Vectashield Mounting medium Vector Laboratories Cat# H-1000 Metal Enhanced DAB Substrate Kit Thermo Scientific Cat# 34065 Dnase I Invitrogen Cat#18068015 Dynabeads G ThermoFisher Cat#10003D Critical commercial assays TruChip Chromatin Shearing Kit Covaris Cat#520154 Human Chemokine Array Abcam Cat#ab169812 Rnascope Multiplex Fluorescent v2 ACD Cat#323110 RNeasy Mini Kit Qiagen Cat#74106 RNeasy Plus Micro Kit Qiagen Cat#74034 GoScript Reverse Transcription System kit Promega Cat#A5001 PrimeScriptTM RT Master Mix Takara Bio Cat#RR036A RealQ Plus Master Mix Green Ampliqon Cat# A324406 Paraformaldehyde (PFA) VWR Chemicals Cat#9713-1000 PierceTM Peroxidase Suppressor Thermo Scientific Cat#35000 Collagenase Sigma-Aldrich Cat# C9407-1G Deposited data E15.5 RNA sequencing This paper GEO: GSE316458 Super-enhancer annotation, Smca1, CTCF, Med1 Chip-seq on human islets of Langerhan DOI: 10.1038/s41588-019-0457-0 EGA: EGAS00001002917 ATAC seq of Panc-1 cells DOI: 10.1186/s13045-021-01131-0 GEO: GSE149103 H3K27ac and H3K4me3 Chip-seq on Panc-1 cells DOI: 10.1038/nature11247 (SYDH HistoneP, ENCODE) GEO:GSM818826 (Continued on next page) 18 Cell Reports 45, 117176, April 28, 2026 ..

    RNA Sequencing:

    Article Title: Regulatory elements in the Sox9 locus license the initiation of pancreatic ductal adenocarcinoma.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER hGastrin I Sigma Aldrich Cat#G9145 mNoggin Preprotech Cat#250-38-100UG N-acetylcysteine Sigma Aldrich Cat#A9165 Nicotinamide Sigma Aldrich Cat#N0636 Primocin Invivogen Cat#iant-pm-2 hR-spondin 3 Life Technologies Cat#120-44-1MG FGF10 Preprotech Cat#100-26 mR-Spondin-1 R&D Systems Cat#3474-RS DNAseI from bovine pancreas Sigma Aldrich Cat#11284932001 Soybean trypsin inhibitor Sigma Cat# T6414-6X100ML Fetal Bovine Serum Gibco Cat#17964671 PenStrep Gibco Cat#15-140-122 Lipopolysaccharide (LPS) Enzo Life Sciences Cat#581-013-L001 Phorbol 12-myristate 13-acetate (PMA) Thermo Fisher Scientific Cat# P8139-1MG NutriStem hPSC XF Medium Biological Industries Israel Beit-Haemek Ltd. Cat#05-100-1A Accutase Thermo Fisher Scientific Cat#A1110501 TrypleE Thermo Fisher Scientific Cat#12604013 Dispase Sigma-Aldrich Cat#D4693-1G ROCK inhibitor Y-27632 VWR Cat#688000-100MG H&E Abcam Cat# ab245880 Alcian blue Vector Laboratories Cat#H-3501 Entellan EMD Millipore Cat#1.07960.0500 Vectashield Mounting medium Vector Laboratories Cat# H-1000 Metal Enhanced DAB Substrate Kit Thermo Scientific Cat# 34065 Dnase I Invitrogen Cat#18068015 Dynabeads G ThermoFisher Cat#10003D Critical commercial assays TruChip Chromatin Shearing Kit Covaris Cat#520154 Human Chemokine Array Abcam Cat#ab169812 Rnascope Multiplex Fluorescent v2 ACD Cat#323110 RNeasy Mini Kit Qiagen Cat#74106 RNeasy Plus Micro Kit Qiagen Cat#74034 GoScript Reverse Transcription System kit Promega Cat#A5001 PrimeScriptTM RT Master Mix Takara Bio Cat#RR036A RealQ Plus Master Mix Green Ampliqon Cat# A324406 Paraformaldehyde (PFA) VWR Chemicals Cat#9713-1000 PierceTM Peroxidase Suppressor Thermo Scientific Cat#35000 Collagenase Sigma-Aldrich Cat# C9407-1G Deposited data E15.5 RNA sequencing This paper GEO: GSE316458 Super-enhancer annotation, Smca1, CTCF, Med1 Chip-seq on human islets of Langerhan DOI: 10.1038/s41588-019-0457-0 EGA: EGAS00001002917 ATAC seq of Panc-1 cells DOI: 10.1186/s13045-021-01131-0 GEO: GSE149103 H3K27ac and H3K4me3 Chip-seq on Panc-1 cells DOI: 10.1038/nature11247 (SYDH HistoneP, ENCODE) GEO:GSM818826 (Continued on next page) 18 Cell Reports 45, 117176, April 28, 2026 ..

    Fluorescence:

    Article Title: Histone Density and Dynamics Shape Mitotic Chromatid Architecture in Xenopus Egg Extracts
    Article Snippet: .. After being counter‐stained with DAPI, the coverslips were mounted on slides with VectaShield mounting medium (Vector Laboratories, H‐1000), sealed with nail polish, and processed for fluorescence microscopy. .. Fluorescence microscopy was carried out using a DeltaVision microscope system (Cytiva), which consisted of an inverted fluorescence microscope (IX71, Olympus) equipped with a UPlanSApo 100×/1.40 oil‐immersion lens and a sCMOS camera (pco.edge 4.2 m, PCO AG).



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